EFFECT OF GROWTH MEDIUM AND INORGANIC CARBON SOURCE ON GROWTH AND BIOMASS COMPOSITION OF TETRASELMIS STRIATA
Patrinou V.1*, Patsialou S.1, Vayenas, D.V.2, Aggelis, G.3, Tekerlekopoulou A.G.1
1 Department of Sustainable Agriculture, University of Patras, G. Seferi 2, 30100, Agrinio, Greece
2 Department of Chemical Engineering, University of Patras, 26500, Rio-Patras, Greece
3 Department of Biology, University of Patras, 26500, Rio-Patras, Greece
Abstract
Tetraselmis striata was cultivated in drilling water with a salinity of 2.8±0.1%. Drilling water was supplemented with two different growth substrates, a ready-to-use commercial fertilizer (Nutri-Leaf 30-10-10) and a modified chemical medium (F/2). Both growth substrates were studied with the addition of NaHCO3 as the only carbon source, with pure CO2 (100% v/v) (flow rate of 1 mL CO2 min−1) or with pure CO2 (100% v/v) (1 mL CO2 min−1) and NaHCO3, simultaneously. The effect of growth medium and inorganic carbon source was then examined on microalga’s growth and biomass composition. The most favorable growth conditions were found to be Nutri-Leaf 30-10-10 and CO2 flow rate of 1 mL min−1 + NaHCO3, which lead to a maximum biomass productivity of 154 mg L−1 d−1 (corresponding specific growth rate of 0.477 d−1) and a significant CO2 biofixation rate (RCO2) of 293.9 mg L−1 d−1. At the same conditions the biomass of T. striata presented the highest quality (proteins 45.1% d.w., lipids 29.0% d.w., carbohydrates 16.1% d.w. and total chlorophylls 5.1% d.w.), while fatty acid analysis also revealed high percentages of eicosapentaenoic acid (EPA up to 18.2% of the total lipids). Therefore, Nutri-Leaf 30-10-10 and CO2 flow rate of 1 mL min−1 + NaHCO3 can be considered a promising strategy for the cultivation of T. striata with the purpose of producing alternative aquafeeds.
Keywords: Growth substrate, inorganic carbon sources, high added value products, fatty acid composition
*Corresponding author: Patrinou Vasiliki (patrinou.v@upatras.gr)
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Posted: 13 Ιουνίου, 2024 by atekerle
5th International Congress on Applied Ichthyology, Oceanography, and Aquatic Environment (HydroMediT 2024)
EFFECT OF GROWTH MEDIUM AND INORGANIC CARBON SOURCE ON GROWTH AND BIOMASS COMPOSITION OF TETRASELMIS STRIATA
Patrinou V.1*, Patsialou S.1, Vayenas, D.V.2, Aggelis, G.3, Tekerlekopoulou A.G.1
1 Department of Sustainable Agriculture, University of Patras, G. Seferi 2, 30100, Agrinio, Greece
2 Department of Chemical Engineering, University of Patras, 26500, Rio-Patras, Greece
3 Department of Biology, University of Patras, 26500, Rio-Patras, Greece
Abstract
Tetraselmis striata was cultivated in drilling water with a salinity of 2.8±0.1%. Drilling water was supplemented with two different growth substrates, a ready-to-use commercial fertilizer (Nutri-Leaf 30-10-10) and a modified chemical medium (F/2). Both growth substrates were studied with the addition of NaHCO3 as the only carbon source, with pure CO2 (100% v/v) (flow rate of 1 mL CO2 min−1) or with pure CO2 (100% v/v) (1 mL CO2 min−1) and NaHCO3, simultaneously. The effect of growth medium and inorganic carbon source was then examined on microalga’s growth and biomass composition. The most favorable growth conditions were found to be Nutri-Leaf 30-10-10 and CO2 flow rate of 1 mL min−1 + NaHCO3, which lead to a maximum biomass productivity of 154 mg L−1 d−1 (corresponding specific growth rate of 0.477 d−1) and a significant CO2 biofixation rate (RCO2) of 293.9 mg L−1 d−1. At the same conditions the biomass of T. striata presented the highest quality (proteins 45.1% d.w., lipids 29.0% d.w., carbohydrates 16.1% d.w. and total chlorophylls 5.1% d.w.), while fatty acid analysis also revealed high percentages of eicosapentaenoic acid (EPA up to 18.2% of the total lipids). Therefore, Nutri-Leaf 30-10-10 and CO2 flow rate of 1 mL min−1 + NaHCO3 can be considered a promising strategy for the cultivation of T. striata with the purpose of producing alternative aquafeeds.
Keywords: Growth substrate, inorganic carbon sources, high added value products, fatty acid composition
*Corresponding author: Patrinou Vasiliki (patrinou.v@upatras.gr)
Full text
Poster
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